Efficiently generate mRNAs

When you want instant expression after transfection into cells, deliver your gene-of-interest as an mRNA made with SBI’s mRNAExpress™ mRNA Synthesis Kit. Designed to generate in vitro transcripts for transfection of mammalian cells, micro-injection into oocytes, in vitro translation, and other related applications, this high-yield kit can produce 20-40 µg of high-quality mRNAs in one standard reaction, and comes with a number of performance-enhancing features:

• Leverages the robust T7 RNA Polymerase
• Incorporates an anti-reverse cap analog (ARCA) into the transcript to increase translation efficiency
• Reduces host cell immune response and enhances stability by incorporating modified nucleotides (5-Methylcytidine-5"-Triphosphate and Pseudouridine-5"-Triphosphate) and a poly-A tail
• Degrades the DNA template after RNA synthesis with the included DNase
• Removes the 5" triphosphates at the end of the RNA with the included phosphatase to further reduce innate immune responses in mammalian cells
• Employs a robust clean-up spin column system that delivers high yields of mRNAs that are ready for most downstream applications

The Kit includes the following reagents that are critical in vitro transcription:

• pMRNA^XP mRNAExpress Vector so you can clone and then transcribe your cDNA-of-interest
• Sequencing primers to confirm the identity of the cDNA insert cloned into the pMRNA^XP Vector
• Tail PCR primer mix to add a poly-A tail to the DNA template
• In vitro transcription reagents—T7 RNA polymerase, in vitro transcription buffer, NTP/Cap mix, RNase-free DNase, phosphatase buffer and RNase-free phosphatase
• Clean-up spin column system, including binding, wash, and elution buffers, collection tubes, and collection tubes with filter cartridges

美国SBI代理 System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒 美国SBI代理 ExoQuick and ExoQuick-TC Products Product Size Catalog # ExoQuick serum exosome precipitation solution (5 ml) 75 reactions EXOQ5A-1 ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product 75 reactions EXOQ5TM-1 Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product 100 reactions TMEXO-1 ExoQuick serum exosome precipitation solution (20 ml) 300 reactions EXOQ20A-1 ExoQuick-TC for Tissue Culture Media and Urine (10ml) 10 reactions EXOTC10A-1 ExoQuick-TC for Tissue Culture Media and Urine (50ml) 50 reactions EXOTC50A-1 Exosome-depleted FBS Media Supplement - USA Certified 50 ml EXO-FBS-50A-1 Exosome-depleted FBS Media Supplement - USA Certified 250 ml EXO-FBS-250A-1 ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit 5 reactions EXOLP5A-1